Expression and characterization of a novel highly glucose-tolerant b-glucosidase from a soil metagenome
نویسندگان
چکیده
Jian Lu1, Liqin Du1, Yutuo Wei1, Yuanyuan Hu1, and Ribo Huang1,2* State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530005, China National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning 530004, China These authors contributed equally to this work. *Correspondence address. Tel: þ86-771-3235706; Fax: þ86-771-3238107; E-mail: rbhuang@gxas.ac.cn
منابع مشابه
Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome.
A β-glucosidase gene unbgl1A was isolated by the function-based screening of a metagenomic library and the enzyme protein was expressed in Escherichia coli, purified, and biochemically characterized. The enzyme Unbgl1A had a Km value of 2.09 ± 0.31 mM, and a Vmax value of 183.90 ± 9.61 μmol min(-1) mg(-1) under the optimal reaction conditions, which were pH 6.0 at 50°C. Unbgl1A can be activated...
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تاریخ انتشار 2013